What is B Cells and key Features of Single B Cell Sorting

by GenScript ProBio Gene and Cell Therapy Vector

What are Single B Cells?

B cells or B lymphocytes are a form of white blood cells that play an essential role in the production of antibodies that aid in the human immune system. B cells cooperate with T cells as well as the other immune system to fight pathogens that cause harm to the body. They play a crucial part in the formation for long-term immune system.

Key Features of Single B Cell Sorting Platform

Ultra-high-throughput screening of B cells for antibody selection

Unprecedented Single-cell resolution

Ideal for complex and critical target cells

Available for all species

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Types of B Cells Sorting

There are four major kinds of B cells: plasma, transitional and memory that each have their own role during the maturation process.

Transitional B Cells

A B cell that is a transitionalis an intermediate between mature B cells that are maturing and immature. They are not able to take actions to protect against disease-causing pathogens, they move between bone marrow as well as secondary lymphoid tissues. In this period they undergo tests to ensure that they do not create autoantibodies which are antibodies which are able to attack the host.

Transitional cells aren't often targeted during B cell separation since they don't have the same role as the other. The study of them within the body can be beneficial enough.

Naive B Cells

The naive B cell is the next stage after the transitional B cells. Once the B lymphocyte matures whether in bone marrow or other lymphoid organs remain a blood cell that is naive until activated.

The process of activation takes place when a mature B-cell is exposed to antigen-presenting cells that are specific to its B receptor. When activated, a naive B cell could transform into the plasma-type B cells, or a memory cell. The cells that are naive don't fight off infection They simply wait for activation by T cells or an antigen-presenting cells (APC).

B cells of the Naive can be examined as well as activated and controlled by using biochemicals that aid researchers gain insight into our immune system. They possess distinct regulatory markers from other cells, which allows their isolation. For example, naive cells are regulated through the CD19 ⁺ CD27 ^- and CD38 ^(-)..

Memory B Cells

A key B cell variant includes one called the memory B cells A type of B lymphocyte vital for the creation of long-term immunities in the body. They remain in the bloodstream even after an infection has gone away. If the host is exposed to the same antigen in the future memories B cells could rapidly activate, with the aid of T cells.

Through the addition of antibodies through memory B cell the immune system may combat pathogens of familiarity before an infection is symptomatic. Memory B cells possess three markers: a CD19 ⁺ +, an CD27 ⁺ and the CD38 ^(-) marker. The distinct expression of the CD27 marker allows scientists to differentiate memory cells and naive cell with just one biomarker.

Plasma B Cells

Plasma B cells often referred to as B cells that effect are massive cells that possess a huge endoplasmic-reticulum ( ER). It is the ER is responsible in assisting the production of and transport proteins. This structure permits plasma cells to create huge amounts of antibodies that are specific to antigens.

The plasma cells react to the signal chemical substances released by T cells in the course of an infection. They continue to produce antibodies to fight off the disease until it is controlled or eradicated. Plasma cells are often present at the site of chronic inflammation.

The plasma B cell is like memory B cells, but they have the CD38 ⁺ biomarker. Since this marker is hard to identify, researchers typically sort B cells in plasma using FACS and the flow cytometer. With three different lasers, scientists can identify all three distinct cells (naive memory, naive and plasma) simultaneously with high-throughput. The effectiveness of this method can be greatly enhanced by enriching the sample with B cells (using the method of preparation similar to BACS) prior to separating these B cells in subtypes using FACS.