How To Get DNA From Dried Blood Spots

Analyzing DNA and recovering genetic material from dried blood spots is crucial for several medical fields. Having high-quality and stable samples of dried blood is vital to extract DNA and achieve results, regardless of the medical discipline. Although blood samples are collected for DNA testing, their collection, storage, and transportation can be challenging. Stable and viable DNA samples are extracted from dried blood instead. Researchers and lab technicians often buy Dried Blood Spot DNA Isolation kits for purifying DNA from dried blood spots.

Blood samples collected and dried on paper cards are gaining popularity because sampling methods can be performed outside care facilities with capillary punctures, and samples can be transported safely. Many hospitals maintain archives of dried blood samples and keep them stored on collection cards. These samples are an excellent source of information to study disease and observe changes over time in individuals and particular areas.

White blood cells are a primary source for extracting DNA because cheek cell samples, which are an alternative source, carry a risk of contamination by bacteria, viruses, and environmental elements. That said, blood samples have a few limitations. Specimens become unusable when they are transported and stored correctly. DNA extraction from wet blood is possible; however, it’s a complex process with time and temperature constraints.

On the other hand, dried blood can be collected efficiently, stored without the need for refrigeration, and later used for DNA extraction. Another benefit of using dried blood spots is DNA’s longevity in dried blood. Several months-old samples show only slight variation due to changes in temperatures and humidity. Studies show no signs of DNA degradation when samples are kept at 4°C for a day, regardless of the extraction method.

Dried blood spot samples are alternative to plasma and serum in instances when there is no expert to take venous blood samples or when transport of body fluids is challenging. Dried blood samples are also an alternative to rapid diagnostics tests. It’s possible to confirm infection with molecular tests and Western blot by using dried blood sample testing of many people.

The first step for DNA isolation from dried blood spots is to rehydrate the sample and separate the blood from the paper. Researchers or lab technicians generally take only a small punch from the sample and place it in a buffer where it is agitated and allowed to soak at high temperatures. It will enable blood cells to resuspend in the buffer. Next, a proteinase and lysis buffer is added to open the cells and break any nucleases. After another incubation session, the sample shows free nucleic acids and cellular debris. It’s best to buy a Dried Blood Spot DNA Isolation Kit to make the process easier because it contains all the required reagents and extraction columns.

Author’s Bio – The author is an online blogger. This article is about getting DNA from dried blood

Sponsor Ads

• Ad Space Available - Rent it Now!

Report this Page

Created on Oct 3rd 2022 00:10. Viewed 92 times.

Share on APSense

Comments