Dos And Don’ts Of Working With RNA Extraction

RNA or ribonucleic acid is a long linear polymer of nucleotides found in the nucleus of a cell where it is associated with granules. RNA extraction is essential for obtaining samples for various sequencing and pathogen testing applications. RNases are present everywhere in every object and microorganisms in the air. Performing RNA extraction is a complicated job because it can contribute to contamination and degradation when mishandling samples. As a part of the precautionary steps for working with RNA, consider the following Dos and Don’ts in the process. 

Dos

Wear a protective suit and gloves

If you are a lab technician, getting close to those contagious things and handling them to execute their tasks is obvious. The job requires handling pathological items such as pathological cells, tissue, bacteria, and other fungal materials. Contacting them through your skin can contaminate the preps or infect you with highly contagious substances. Before starting the experiment, you should wear hazmat suits, gloves, masks, caps, and goggles. 

Choose suitable pipettes and containers

To conduct a test on a biological sample, you need to invest in containers such as beakers, test tubes, flasks, graduated cylinders, wash bottles, and funnel. These containers can be of glass or plastic, depending on the type of experiment requirements. If glassware is too expensive, look for consumable laboratory items that are durable and less expensive as compared to glass. 

Get reagents

When transferring a substance into the test tube, decontaminating it using an RNase solution is essential. The reagents should be of RNase-free water that works well for RNA extraction. Find the biochemical supplier that offers Auto-Mag RNA clean-up kit shipping service in your area. The top-quality product comes with a magnetic beads-based reagent for the RNA clean-up procedure, requiring no centrifugation or filtration before you use it.

Don’ts

Touching things with bare hands

Many pathological samples and chemical components do not produce flame and smoke, but some have a quick reaction when touching another element. You could get infections if you accidentally touch an object with RNA or inhale it. Wearing facemasks and goggles is mandatory for handling those elements during the experiment. Do not talk, cough, sneeze, or breath over open containers, and keep them closed before taking up the next task.

Mixing with incompatible chemicals

After completing the test, you want to clean the pipettes before leaving, but rushing can cause grave mistakes. Someone new to the practice could rinse the tissue stored in the RNase inhibitor. While doing so, mixing the substance with detergent or other chemicals is the worst decision. In case of contact with the substance, do not delay calling for help. Make sure the lab facility has the proper layout for furnishing so you can move freely without grazing hazardous materials.

If you are looking forward to manual and automation-friendly sample processing, purchase an automated RNA clean-up kit and guidelines for adapting the kit.

Author’s Bio: The writer is an avid online blogger. This article is about Auto-Mag RNA Clean-up Kit shipping.