Cu free click chemistry and its various reagents

Click chemistry

Click chemistry is the study of diverse reagents for understanding their properties & applications. It is fundamentally defined to be an approach for straightforward, efficient and precise labeling & finding of bio-molecules. A case of point is DBCO-TAMRA, a reagent for use in sample labeling, conjugation & derivation method.

Difference between customary and new Click Chemistry

Customary "Click Chemistry" have need of a Cu(I) catalyst which is poisonous to the majority of organisms and so, prevent it from being made use of in lots of biological systems.

The fresh Copper-free Click Chemistry happens to be based on reaction between a DBCO moiety and a reaction partner thatis azide-labeled, identified as strain-promoted SPAAC. This fresh form of "Click Chemistry" is incredibly speedy at room temperature and has no need for a cytotoxic Cu(I) catalyst. Cyclooctynes happen to be thermostable and has with very selective and precise reactivity toward azides, and this result in nearly quantitative production of stable triazoles.

This technique has need of the activation of the biomolecule #1 by means of DBCO reagent, and the biomolecule #2 by means of azide and the subsequent mixing of the couple of activated biomolecules for forming a conjugate.

Features & benefits

Biocompatibility – has no need for cytotoxic Copper catalyst – good for in-vivo applications.

Gentle conditions – conjugation in an aqueous buffered media & at low temperature

Stability – DBCO & azide moieties happen to be enduringly stable

Effectiveness – quantitative production of a stable triazole

Specificity & Bioorthogonality – azide can react with DBCO even when -SH, -NH2, -COOH & added protein functionalities are present

The products of Cu free Click Chemistry consist of:

•    DBCO reagents

•    Chemical Modifications Reagents containing DBCO for introducing functional groups like carboxylic acids, amines, / NHS esters

•    Biotinylation Reagents containing DBCO with different spacers for introducing Biotin moieties

•    Spacers containing DBCO -Spacer & linker building blocks consisting of a DBCO moiety

•    Fluorescent Dyes containing DBCO - different fluorescent dyes adjusted with a DBCO group for joining with azides

•    Nucleotides containing DBCO - Nucleotides consisting of a DBCO moiety for joining with azides by the use of Copper free click reactions

List of Reagents containing DBCO for Cu-free Click Reactions

DBCO-Amine

Dibenzylcyclooctyne-Amine

DBCO-Acid

Dibenzylcyclooctyne-Acid

DBCO-NHS ester

DBCO-PEG4-NHS Ester

Dibenzylcyclooctyne-NHS ester

DBCO-S-S-NHS ester

Dibenzylcyclooctyne-S-S-NHS ester

DBCO-Maleimide

Dibenzylcyclooctyne-Maleimide

Sulfo-DBCO-NHS ester

Sulfo-Dibenzylcyclooctyne-NHS ester

Sulfo-DBCO-NHS ester

List of Biotinylation Reagents containing DBCO

DBCO-Biotin Conjugate

Dibenzylcyclooctyne-Biotin Conjugate

DBCO-PEG4-Biotin Conjugate

Dibenzylcyclooctyne-PEG4-Biotin Conjugate

DBCO-PEG12-Biotin Conjugate

Dibenzylcyclooctyne-PEG12-Biotin Conjugate

DBCO-S-S-PEG3-Biotin Conjugate

Dibenzylcyclooctyne-S-S-PEG3-Biotin Conjugate

DBCO-S-S-PEG11-Biotin Conjugate

Dibenzylcyclooctyne-S-S-PEG11-Biotin Conjugate

Sulfo-Dibenzylcyclooctyne-Biotin Conjugate

Sulfo-DBCO-Biotin Conjugate

NHS Ester of Biotin

NHS Ester of Biotin makes Biotin labeling of proteins & any additional key amine-containing macromolecules. Such biotinylated molecules happen to subsequently be detected by the use of fluoresecent or HRP-labeled streptavidin. Biotoc NHS ester reagents available are UV-Tracer™ Biotin-NHS and Biotin-PEG4-NHS ester

Desthiobiotin-PEG3-Azide

This is an desthiobiotin labeling reagent that is azide activated and has a hydrophilic spacer arm.

About Author

DBCO-PEG4-Biotin reacts with azides via a copper-free "click chemistry" reaction to form a stable triazole. Expand the boundary of your discovery research with our click chemistry toolbox. Find more information about DBCO-PEG4-NHS Ester.