About DBCO TAMRA

There are a lot of staining dyes that are made available by the scientists. These dyes play a significant role in making the biomolecules labelled. The labelling of biomolecules is essential because it makes it easier for the chemist to locate and detect the proteins that are to be used. Moreover, all dyes differ in qualities from other dyes. Every cell has to stain using a special group of dyes so that no adverse reactions may take place. DBCO TAMRA is one of the dyes that is used for the labelling purposes. Mostly it is available in powder for. The powder form is very advantageous because the powder can be dissolved in any type of solvent.

Storage Temperature

For the labelling chemicals, it’s essential that they are stored under optimum conditions. If they are not stored in optimum conditions and under controlled temperature then there is a risk that the might react with the surrounding elements or substances and cause serious damage. Therefore, it is necessary that these chemicals are stored under controlled temperature and controlled conditions. The optimum temperature for storing the DBCO TAMRA is up to -20 degree Celsius.

Solubility of the Dye

The solubility of the dye is another factor that you need to consider before staining the biomolecules. There are different types of solvents in which the dyes can be dissolved. These are polar and non-polar solvents. The compounds made largely of organic compounds are dissolved in polar solvents. The DBCO TAMRA is soluble in organic solvents like DMSO, DMF.

Chemoselectivity

The Chemoselectivity and Biocompatibility are the two factors mainly responsible for the dye to work properly. Chemo Selectivity is the property of the dye to react with different solutes and solvents. The DBCO TAMRA reacts with only those chemicals that have a high efficiency for the reaction. Otherwise, it remains stable during the whole procedure but you should take care while the labelling process is being carried out.

Biocompatibility

Biocompatibility is the term that is related to the composition of the dye. Some dyes don’t work properly if not treated with some kind of chemicals. Some chemicals need certain reagents and solvents for their proper working. The DBCO TAMRA is one the best dues that is biocompatible. It does not require other chemicals and reagents to make it work properly.

Reaction of DBCO TAMRA

The TAMRA dyes and labels easily react with the azides. Their reaction is called as the copper free reaction. As a result, of the reaction, a stable triazole is formed. The reaction does not require the Cu- Catalyst to take part in the reaction. The dye gives high shades in the backgrounds so the permeable cells must not be stained.

Staining the Intracellular components

The DBCO TAMRA is not suitable for staining the intracellular components. The cells that have a higher degree of permeability are not advisable to be stained with this dye because it can make higher backgrounds that are not useful for the chemists. The intracellular components are kept safe from staining.

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DBCO TAMRA is one of the dyes that is used for the labelling purposes. Then don’t forget to check out Click Chemistry Reagents.