Advantages of Cy5 Azide and Cy5 Tetrazine

The chemical is used widely by the chemist in dying procedures. It is has a vast use in dying of proteins, amino groups and DNA’s etc. it is mostly available in a 10mM solution in DMSO. The dye has an infrared emission. The labelling chemical is used for imaging the organism.

Appearance

Mostly the labelling chemicals are in solid powder from. The powder form makes it easier for the chemicals to mix up in solvents. The Cy5 picolylAzide exists in the powder form and also available in solution form. The powder is dark blue in colour, which is the characteristic colour of Cy5 PicolylAzide.

Molecular Weight

The molecular weight of Cy5 PicolylAzide is around 701.34. The molecular weights of the labelling chemicals are very important in determining their characteristic properties.

Molecular Formula

The labelling chemical Cy5 PicolylAzide is primarily made up of carbon (C), hydrogen (H), chlorine (Cl), nitrogen (N) and oxygen (O). The chemical comprises of C = 34, H = 49, Cl = 1, N = 6, O = 1.

Solubility

The chemical is soluble in organic solvents. As the compound consists of organic elements so it is soluble in organic solvents. Some of these solvents are DMSO, DMF, dichloromethane etc. the chemical ha low solubility in water because water is a polar solvent.

Storage Conditions

Specific storage conditions are required to store such chemicals because these are highly reactive and need precise conditions so that they don’t react to the surroundings. Desiccation is the best technique to store such powders so that their reactivity can be decreased. Moreover, a temperature of -20 degree Celsius is optimum for their storage.

Absorption and Emission Spectra
 
The absorption and emission spectra of the labelling chemicals are essential. These spectra depict the nature of the protein or amino group that has been labelled. Moreover, they are used to detect the nature of proteins. The absorption spectrum ranges from 660 to 690 nm. And the emission spectrum ranges from 700-710 nm.

About Cy5 Tetrazine

This is a kind of fluorescent probe that is widely used for chemo selective labelling. This is also used in detecting the TCO-tagged molecules.

Chemo Selective

The chemo selective ability of the chemical makes it very useful. Due to its chemo selective ability, it does not react with any other biological sample placed in the solution but only reacts with the molecules that have a relatively higher efficiency with it.

Biocompatibility

The biocompatibility of the chemicals makes them very useful and advantageous. Those elements are termed as biocompatible that react or use p in their original form and don’t require any other chemicals for their action. Likewise, the Cy5 Tetrazine is biocompatible it does not require any reducing agents for their action. The Tetrazine-activated fluorescent dyes are very well suited for the detection of TCO labelled molecules and biopolymers. The chemical possesses excellent reaction rates. Its reaction kinetics is suitable for every reaction. It has an inverse-electron-demand Diels-Alder chemistry.

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