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Forest plots were constructed for each comparison subgroup. Each individual trial effect size is shown with confidence intervals as a box whose area is inversely proportional to the estimated variance of the effect in that trial. The pooled estimate and its confidence interval are shown as a diamond at the bottom of the plot with a dotted vertical line indicating the pooled estimate value. A vertical solid line at zero indicates no treatment effect.

For each trial, we calculated the monthly weight loss percentage for each treatment group and the placebo group. Monthly weight loss percentage is defined as the mean monthly weight loss divided by the mean baseline weight in that group. Unfortunately, we were not able to

calculate monthly weight loss percentage on an individual level. To determine the standard 21 deviation of the monthly weight loss percentage, we used the delta method100 and assumed a correlation of 0.5 between the baseline and follow-up weights.101 For each comparison subgroup, we pooled monthly weight loss percentages in Choice 9000 Caralluma the treatment groups and placebo groups separately using a random effects model99 and produced associated 95% confidence intervals.

We acknowledge that combining estimates within treatment groups only, or placebo groups only, does not take advantage of the randomization and pairing of treatment and control within a trial.

This lack of pairing, and the fact that the monthly weight loss percentage in the treatment group must be compared to the associated monthly weight loss percentage in the placebo group, should be kept in mind when interpreting the results of this analysis.

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