Articles
RANGE OF SILICA GEL MESH FOR CHROMOTOGRAPHY
by Priya Singh An Impurity Free Chromatography for Higher Yield
Discovery of Chromatography technique has made a revolution
in the field of analytical laboratories for the separation of the chemicals
after synthesis. Since the separation process for each compound is different
based on their physical interactions with the solute and the absorbent, different
methods were discovered for an efficient and reliable analysis. Since for any
chromatographic technique, the absorbent remains the same i.e. Silica Gel adsorbent, because it is low
acidic and available in definite particle size ranging from 60-800 mesh size.
There are many Chromatography techniques theoretically, but only few can be
operated practically in the laboratories. Among these techniques Column
chromatographic techniques are mostly preferred as it is low in cost as well as
requires minimum instrumentation. In
turn based on the flow of the solvent down the column through the absorbent,
Column chromatography is classified into Gravity Column Chromatography and
Flash Chromatography. The flow of the solvent in the column is based on the
definite pore size of the Silica Gel, which allows smaller molecules into its
matrix and thereby excludes the larger molecules which flow down and get eluted
faster.
Silica Gel Mesh for
Chromatography.
If the solvent flows down the column passing the Silica gel
absorbent of definite particle size by gravity or fissure, it is referred to as
Gravity Column Chromatography. But if the solvent flows down the column passing
the Silica gel absorbent of definite particle size by positive air pressure, it
is referred to as Flash Chromatography. Therefore the absorbent used in both
these techniques is the same, the Silica Gel Mesh. There are many products of
Silica Gel mesh available depending on the mesh size such as Silica Gel 60-120 mesh, Silica Gel 60-200 mesh, Silica Gel 70-230 mesh, Silica Gel 200-400 mesh and Silica Gel 400-800 mesh. Among this
Silica Gel 70-230 mesh is used for Gravity Column Chromatography and Silica Gel
200-400 mesh is used in Flash Chromatography. These all are available in a thin
white free flowing powder form with a density of 0.75 gm per ml. Best separation is achieved in Ordinary
compounds by Silica Gel Absorbent. Apart from Gravity Column Chromatography and
Flash Chromatography, there is another technique in Chromatography named as
Reversed-phase Chromatography, which is employed for the solvent which is more
polar than the Absorbent. Silica gel was the first polymer used formerly in
Reversed-phase Chromatography for the purification of small organic molecules
but later for the purification of synthesized Peptides. The separation is
achieved by the partitioning mechanism between the Stationary phase and the
mobile phase in Reversed-phase Chromatography, in which the stationary phase is
Silica Gel.
Overall, in each and every Chromatographic techniques the
main absorbent is the Silica gel and is considered as the best absorbent in the
analytical field, as it is porous, insoluble
and its hydrophobic interaction with the solvent makes the elution process
progress at a faster rate as the polar components get eluted first. These
Silica gels exist in different particle sizes, because of which they are used
in many Column Chromatography Techniques and Sorbead being the best Silica gel 200-400 supplier and Silica gel 70-230 mesh supplier.
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About Priya Singh An Impurity Free Chromatography for Higher Yield
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Joined APSense since, January 7th, 2015, From Vadodara, India.
Created on Sep 4th 2017 02:22. Viewed 454 times.
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