Pig Gallbladder powder agreeable assurance

Take Pig acerbity  powder of about 0.1g, accurately weighed, with 10% sodium hydroxide band-aid 5ml, 120 ℃ for 4 hours, cooled to agitate with a band-aid of hydrochloric acid to acclimatize the pH to 2-3. With ethyl acetate extracted four times, anniversary 10ml, the accumulated extracts were evaporated and the balance was attenuated in ethanol, transferred to a 10 mL volumetric flask, add booze to the mark, shake, as the analysis solution.

More accurately advised controlled beneath bargain burden for 24 hours hyodeoxycholic bulk of phosphorus pentoxide dryer, add booze per 1 ml band-aid absolute 1 mg is able as a advertence solution. Attenuate band chromatography (Appendix Ⅵ B) test, attention blot the analysis band-aid 1 ul, the advertence band-aid 1 ul and 3 ul anniversary of the circle in the aforementioned silica gel G plate, afresh able isooctane - ether - ice Acetate - butanol - baptize (10: 5 : 5: 3: 1) top band-aid as a means, started, dried, sprayed with 10% sulfuric acid in ethanol.

At 105 ℃ acrimonious to ascertain blush clear, abolish the awning bowl of the aforementioned admeasurement of the bottle to anchored with adhering tape, attenuate band chromatography (Appendix Ⅵ B TLC scanning method) for scanning, wavelength:?; s = 375 nm, lambda R = 650 nm, altitude of the analysis absorbance basic bulk with the advertence actuality assimilation basic bulk for the calculation, that is, too.

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