Major Cell Based & Cell Free Assays In Drug Discovery Biology Servicesby Integral Bioscience drug discovery chemistry services
Drug discovery requires comprehensive expertise from chemistry to biology combining thoughtful strategies and communication with high-quality data. Drug discovery biology services are a significant part of integrated drug discovery that includes an array of assays. These assays help CROs to assess vital information regarding drug compounds.
With wide-ranging capabilities in biology, drug discovery laboratories become able to offer high-quality, seamless, and cost-effective drug discovery services. Let us have a look at some of the major cell-based and cell-free assays that are part of drug discovery biology services along with their purpose.
The word “cytotoxicity” contains two prominent terms, that are, "cyto" which means cell, and "toxic" which means poison. Cytotoxicity refers to the degree to which a drug compound can cause harm to a cell. It can lead to apoptosis (programmed cell death), necrosis (uncontrolled cell death), or stop actively growing and dividing to decrease cell proliferation.
Cytotoxicity assays help drug discovery chemists in India to determine the ability of a drug compound to cause cell damage or cell death. These assays are widely used for research and drug discovery to screen toxic compounds. It proves to be critical to evaluate the effects of a drug compound.
As the term itself is suggesting, an enzyme assay is the laboratory technique that is done in drug discovery laboratories to assess the enzyme activity of the given sample drug compound. Enzyme assays can serve various purposes such as determining the presence of an enzyme, finding specific enzyme kinetics, or the activity of inhibition within the sample compound.
For determining enzyme activity, both qualitative and quantitative approaches are used by drug discovery chemists in India. Qualitative assays are done to confirm the presence of a particular enzyme whereas a quantitative assay is performed to measure the amount of the target enzyme present within a sample drug compound.
ELISA assays or Enzyme-linked immunosorbent assays have been one of the prominent techniques practiced by drug discovery laboratories for several decades to detect the presence of a substance, usually an antigen, in an aqueous sample of a drug compound. In drug discovery projects, a standard single-analyte immunoassay is performed with a plate-based ELISA to detect an antigen or another marker of interest.
With time a multitude of detection chemistries has been applied by the prominent drug discovery chemists in India and across the globe to ELISA, such as chemiluminescence and fluorescence readouts. Recently, bead-based methods and microfluidic platforms have started to improve immunoassays and along with single-molecule detection, the sensitivity of traditional ELISA is attaining new levels.
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Created on Mar 16th 2022 01:40. Viewed 175 times.