What is Inverted Fluorescence Microscope and Where is It Used

An inverted fluorescence microscope is an ideal equipment for quality fluorescence imaging. With an effective lamp fluorescence illuminator, filter, and objective lenses, the microscope helps generate sharp fluorescence images. Learn different types of microscopes for fluorescence images and their uses. 

An inverted microscope has plenty of benefits over an upright one. It gives you more freedom and enables you to quickly test more samples. The equipment can phase live cell imaging and contrast imaging. Also, a variety of accessories can be used with the microscope to extend the device’s usability.      

The inverted microscope comes with a light source and condenser on the top, while its objectives and turret are below the stage, pointing up. It enables a user to image living cells more efficiently. 

Features of an Inverted Fluorescence Microscope 

A modern inverted fluorescence microscope is a quality device that has a fluorescence illuminator and multiple large objective lenses to produce reliable images. It can be paired with a range of accessories to extend the equipment’s usability. 

Also, you can expect features like:

• A wide-field eyepiece to get a wide view and sharp images

• Multiple objective lenses for high-quality imaging

• Comfortable viewing from an inclined angle

• Accessibility with different types of phase contrast accessories for quality results

• Enhanced control through a motorized stage

• Glass stage to prevent stage deformation

• Many petri dish holders for imaging

Applications of an Inverted Fluorescence Microscope 

In an inverted microscope, the stage’s top holds a condenser and a source for transmitted light. Objectives are placed below the stage pointing up. The device has a cell culture vessel, whose bottom is used to observe the cells. 

An inverted microscope’s applications include living cell imaging. Usually, during the process of imaging, cells settle at the bottom of a vessel and spread quickly. It becomes difficult to observe them using equipment other than an inverted fluorescence microscope. 

When the living cells are in a vessel, they have access to a larger medium. Through an inverted microscope, it is easier to image these cells. A quality microscope ensures easy access to objectives. Also, it can be coupled with a range of accessories for more useful outcomes.

How to Buy an Inverted Fluorescence Microscope

The popularity of inverted microscopes has grown dramatically in the last few years. They are perfect equipment for high-quality fluorescence imaging. When it comes to buying a microscope, there are plenty of things to consider, and they include fluorescence filter sets, focusing mechanism, objective lens, eyepiece, etc. 

Apart from that, take a device’s features into account to ensure that a reliable, quality microscope has been chosen for high-quality fluorescence imaging. Check whether or not the machine can be used with various other accessories for the required results.

In the End

An effective, reliable inverted fluorescence microscope makes it easy to produce quality images for tests. Get the best microscope and make some ground-breaking discoveries. All the best!