How to select a protein assay
Protein quantification (procedure for determining the amount of protein) plays a fundamental role in protein research. There are different methods used for measuring protein concentration, with Bradford protein assay being the most popular one. The other protein quantification assay methods comprise bicinchoninic acid, folin-lowry, and kjeldahl. These varying protein assay methods suit different protein types.
Thus, the question is: how does a researcher select the best protein assay method? Is there a guideline that they have to follow to achieve this objective? After all, one cannot deny the significance of choosing the right protein assay to derive optimal research solutions, and as a result, reduce the risk of costs exceeding a researcher’s estimated budget.
While there are no hard and fast rules for selecting protein assays, it is crucial for researchers to select an assay method that is most compatible with a particular protein sample. The best approach to do so is to study and understand different assay methods. This will help researchers analyze the advantages and limitations of different methods.
Remember, there is no single, ideal method for protein concentration. Each method has its own advantages and disadvantages. And no single method gives the same results in varying situations. That is primarily the reason why most researchers have multiple protein assays in their labs.
Analyzing different methods will also researchers the opportunity to assess and formulate a strategy to address the limitations of their preferred assay. As a rule of thumb, any method that would require researchers to make the least amount of changes to a sample would work.
Aside from the protein sample and components, equally important is to choose an assay method based on its range and required sample volume. In addition, protein-to-protein uniformity is another key determining factor. Samples change color when used for different assay methods, and that is what protein-to-protein uniformity is all about.
Similarly, researchers must assess the convenience of various assays. They must, therefore, select an assay that can test a maximum number of samples immediately, without any complications. In addition, through prior testing of a sample under similar conditions, researchers can determine the most effective method for their projects.
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