Top 12 Biochemical Engineering Questions Answered By Experts

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Stop looking through nth book to find the answers to your Biochemical Engineering Questions. As the top12 questions of the field have been answered by the experts here. These experts are biochemical engineering assignment writing service providers, so feel confident with these answers.

Let us, quickly get a glimpse of them:

 

Questions About the World of Microbial

 

Q.1. What is the major difference between gram positive and gram negative cell wall of the bacteria?

 

l  Gram positive: This cell wall consists of multiple layers of peptidoglyclan, without a lipid outer membrane.

l  Gram negative: Here the cell wall has only a few layers of peptidoglyclan along with an outer membrane that has multiple lipid complexes.

 

Q.2. How does the bacteria and eukaryotic cells differ from each other in terms of the genetic material?

 

l  The DNA in prokaryotic cells is generally organized as a simple circular molecule that is placed in the nucleoid region of cytoplasm.

l  Eukaryotic cells on the other end, have their DNA divided in various chromosomes that are located in the nucleus. Also, additional genetic material is found in some organelles like chloroplasts and mitochondrion.

 

Questions About Viruses and Cell Organelles

 

Q.3. Discuss the differences between lytic and lysogenic cycles of viruses.

 

l  Lytic Cycle: Here the host bacterium is destroyed that is, it undergoes lysis. It produces progeny phases and it cannot be converted into lysogenic cycle.

l  Lysogenic Cycle: The bacterium and the phage shares a symbiotic relationship, such that the host is not affected. Here the DNA of the phage becomes a part of the bacterial chromosomes. If exposed to certain chemicals and/or physical agents it can be converted into lytic cycle. These agents may vary from UV rays to nitrogen mustard.

 

Q.4. List the nucleic acids that are found in viruses.

 

l  Depending on the nucleic acid that a virus carries it can be either a DNA virus or a RNA virus. Majorly viruses have RNA genome. Single-stranded RNA viruses are found in plant viruses and double-stranded DNA genomes are found in bacteriophages.

 

Questions About Carbohydrates

 

Q.5. Distinguish the following in terms of linkage.

 

l  Starch and Cellulose: In starch large number of glucose units are joined together by glycosidic bonds. Cellulose consists of some several hundreds to nearly ten thousand β(1→4) D linked glucose units.

l  Lactose and Maltose: Lactose is a disaccharide where one glucose and galactose are bonded through β-1→4 linkage. Whereas, maltose is a disaccharide which uses α (1→4) bond to join two glucose units.

 

 

Q.6. Give three examples of monosaccharides.

 

l  Fructose, Glucose (dextrose), and galactose.

 

Questions About Nucleic Acid

 

Q.7. Differentiate between DNA-B helix and DNA-Z helix.

 

Geometry Attribute	DNA-B Helix	DNA-Z Helix
Helix sense	It is right-handed	It is left-handed
Repeating Unit	1 bp	2 bp
Diameter	20 Å	18 Å
Base per turn	10.5	12
Rise base pair among axis	0.332 nm	0.38 nm

 

Q.8. What are nucleoside and nucleotide?

 

l  Nucleoside= sugar+base. It contains a sugar group and a base.

l  Nucleotide= sugar+base+phosphate. Through phosphorylation of nucleoside by kinases produces nucleotides.

 

Questions About Protein

 

Q.9. Name the techniques through which protein quantification can be done.

 

l  There are various techniques through which protein quantification can be done, this includes:

1) Bicinchoninic acid protein assay,

2) Bradford dye binding protein assay,

3) Colloidal gold protein assay,

4) Lowry protein assay, and

5) Ultraviolet absorption protein assay.

 

 

Questions About TCA Cycle

 

Q.10 What is the cite for TCA cycle? Can it take place in anaerobic system?

 

l  TCA cycle takes place in the matrix of the mitochondrion.

No, TCA cycle can never take place in anaerobic system. As there the pyruvate is converted into ethanol or lactate. Therefore, it has to take place in an aerobic system.

 

 

Questions About Urea cycle

 

Q.11. Calculate the APA equivalents required for following synthesis:

 

l  1 molecule of glucose from 2 molecules of lactate via gluconeogenesis,

 

The net reaction of gluconeogenesis will be:

 

2 Lactate + 4 NADH + 4 ATP + 2 GTP = Pyruvate + 4 NAD + 4 ADP + 2GDP + 6 Pi

Thus, the total number of ATP required is 18 (4 x3 + 4 + 2).

 

 

 

Questions About Biochemical Manufacturing

 

Q.12. Discuss any 3 precautions to consider while handling plant cells.

 

l  The dose of fungicides and antibiotics has to be perfect in order to prevent any contamination.

l  Sterile conditions should be maintained. This includes use of filtered air and glassware serialization.

l  For survival and proliferation of cells optimal maintenance of physical factors is required like- pH level, humidity, lighting, and temperature.

 

With these 12 questions answered, your Biochemical Engineering assignment writing will surely be all sorted.

 

Summary: This article provides answer to the top 12 questions that will help biochemical engineering students to understand the concepts of the field and write an assignment on the same.

 

Author: Marie Claire is a professional writer at Assignment Desk, her assignment assistance has helped many students. In her leisure time, she likes to listen to music.

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Created on May 9th 2019 03:17. Viewed 438 times.

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